Rapid RP-HPLC method was developed for simultaneous estimation of isoniazid, metformin hydrochloride and glimepiride. Analysis was done in synthetic mixtureof isoniazid, metformin and glimepiride. Stationary phase as Hibar® 250-4.6 HPLC column Purosphen® STAR RP-18. Mobile phase methanol:water at the ratio 70:30 v/v was used. Flow rate was 0.8 ml/min, wavelength was set as 234 nm and the volume of inject was 100 ppm. The retention time of the isoniazid, metformin HCl and glimepiride was found to be 2.88, 2.41 and 4.66 minutes respectively.
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